@article{JoossScholzMeixneretal.2019,
  author    = {Kevin Joo{\"s} and Nico Scholz and Jens Meixner and Christian Neus{\"u}{\"s}},
  title     = {Heart-cut nano-LC-CZE-MS for the characterization of proteins on the intact level},
  series   = {Electrophoresis},
  volume    = {40},
  issn      = {0173-0835},
  doi       = {10.1002/elps.201800411},
  pages     = {1061 -- 1065},
  year      = {2019},
  abstract  = {Multidimensional separation techniques play an increasingly important role in separation science, especially for the analysis of complex samples such as proteins. The combination of reversed-phase liquid chromatography in the nanoscale and CZE is especially beneficial due to their nearly orthogonal separation mechanism and well-suited geometries/dimensions. Here, a heart-cut nano-LC-CZE-MS setup was developed utilizing for the first time a mechanical 4-port valve as LC-CE interface. A model protein mixture containing four different protein species was first separated by nano LC followed by a heart-cut transfer of individual LC peaks and subsequent CZE-MS analysis. In the CZE dimension, various glycoforms of one protein species were separated. Improved separation capabilities were achieved compared to the 1D methods, which was exemplarily shown for ribonuclease B and its different glycosylated forms. LODs in the lower μg/mL range were determined, which are considerably lower compared to traditional CZE-MS. In addition, this study represents the first application of an LC-CE-MS system for intact protein analysis. The nano-LC-CZE-MS system is expected to be applicable to various other analytical challenges.},
  language  = {en}
}