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Förster resonance energy transfer - what can we learn and how can we use it?

  • The present manuscript gives a short overview on Förster Resonance Energy Transfer (FRET) of molecular interactions in the nanometre range. First, its principle is described and a short historical overview is given. Subsequently some principal methods and applications of FRET sensing and imaging are described (with some emphasis on fluorescence lifetime imaging, FLIM), and finally two innovative FRET techniques are presented in more detail. Applications are focused on measurements of living cells.

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Metadaten
Author:Herbert Schneckenburger
Institutional Author:Herbert Schneckenburger
DOI:https://doi.org/10.1088/2050-6120/ab56e1
ISSN:2050-6120
eISSN:2050-6120
Source Title (English):Methods and applications in fluorescence
Document Type:Article
Language:English
Year of Completion:2019
Release Date:2021/05/11
Volume:8
Number of Pages:17
Faculty:Optik und Mechatronik
Relevance:peer reviewed