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The present manuscript gives a short overview on Förster Resonance Energy Transfer (FRET) of molecular interactions in the nanometre range. First, its principle is described and a short historical overview is given. Subsequently some principal methods and applications of FRET sensing and imaging are described (with some emphasis on fluorescence lifetime imaging, FLIM), and finally two innovative FRET techniques are presented in more detail. Applications are focused on measurements of living cells.
Light sheet module for 3d imaging – a miniaturized device permits 3d resolution in microscopy
(2018)
Side Views in 3D Live Cell Microscopy – Innovative Concepts of Illumination for Novel Applications
(2020)